contaminating MACs making IL-6 appear to account for Th17 response with IL-21 plus TGFb or TGFb alone-- result varies between T cell preps, probably depends on extent on MAC hyperplasia in KO
check new MAC cultures for IL-23 and TGFb.
does IL-23 account for the lack of inhibition for the KO in the 2° IL6/TGFb culture? check effect of added IL-23 in that assay.
New 4KO and B4KO data suggest no real difference in Th17 response with B4KO-- either with IL6/TGFb alone or with added IL4
does IL-4 exposure prime T cells to be resistant to TGFb?
need to check IL17 in fresh B4KOs
check gamma-delta cells making IL-17 in BKO mice
Subscribe to:
Post Comments (Atom)
No comments:
Post a Comment