Th17 story, continued, and thoughts

contaminating MACs making IL-6 appear to account for Th17 response with IL-21 plus TGFb or TGFb alone-- result varies between T cell preps, probably depends on extent on MAC hyperplasia in KO

check new MAC cultures for IL-23 and TGFb.

does IL-23 account for the lack of inhibition for the KO in the 2° IL6/TGFb culture? check effect of added IL-23 in that assay.


New 4KO and B4KO data suggest no real difference in Th17 response with B4KO-- either with IL6/TGFb alone or with added IL4

does IL-4 exposure prime T cells to be resistant to TGFb?

need to check IL17 in fresh B4KOs



check gamma-delta cells making IL-17 in BKO mice

Th17 story

increased IL-17 from fresh KO T cells

decreased IL-17 under in vitro Th17 differentiation conditions with low TGF-beta

higher TGF-beta or anti-IL-4 Ab restores defect

but what causes increased IL-17 in vivo?

under some conditions, increased IL-17 seen from KO T cells differentiated in vitro

T cell stimulation-- kinetics of cytokine expression

T cells stimulated with plate-bound anti-CD3-- when does cytokine mRNA induction peak?

4 hr does not seem to correlate with cytokine secretion (protein expression levels)

16 hr seems to correlate better with cytokine secretion

Does BCL6 just make T cells phenotypically unstable?

Sometimes I feel that way.

Need to Get a Paper Out

I have a nice story but it's incomplete-- and now I'm getting screwed by the mouse-to-mouse variability in BCL6 KO mice in terms of Th17 responses!

Argghh.