Treg plans

General plan--
we will pursue mechanism for BETTER suppression activity of KO Foxp3+ Tregs; we will NOT pursue mechanism of poor suppression of CD25+ Tregs from KO

General models for better suppression activity of KO Tregs:
1) lack of BCL6 in Tregs leads to intrinsic signaling alteration via TCR
2) KO Tregs are essentially normal but are preconditioned in vivo by IL4 (other cytokines?) to have better activity

Can test the preconditioning effect more easily-- test if WT FoxP3+ Tregs show better suppression activity when pre-incubated with IL-4


Next sorting expt---

sort out WT and KO GFP+ Tregs (following initial enrichment with Macs)
make RNA from resting and 6h stim cells-- test Treg gene expression by QPCR: Foxp3, TGFb, CTLA4, IL35; also IL2, IL4, IL10, IL17.